The Vcheck cCortisol is an in vitro diagnostic test kit for the quantitative measurement of cortisol concentration in canine serum. Cortisol is one of steroid hormone in a glucocorticoid hormone group that are important in the regulation of metabolism. Cortisol is excreted in the adrenal glands in response to stress or low blood sugar concentrations. The Vcheck cCortisol can be used to assist in the monitoring of cortisol related diseases. The Vcheck cCortisol is designed to be used only by veterinarians.
The Vcheck cCortisol Test kit is a fluorescent immunoassay for the quantitative measurement of canine cortisol concentration. The Vcheck cCortisol Test is based on the competitive immunoassay method. When the specimen is deliverd to the sample hole of the test device, cortisol in the specimen and fluorescence micro particles conjugated to cortisol-BSA in conjugate pad migrates along the nitrocellulose membrane. Cortisol in the specimen competes with the Cortisol-BSA for binding sites of the anti-cortisol antibodies coated on the membrane. The density of the test line is inversely proportional to the canine cortisol concentration in canine serum. The BIONOTE Vcheck Analyzer reads the density of the test line and calculates the cortisol concentration from the calibration curve data. The control line is a reference line that indicates the test has been performed correctly.