Canine SNAP® 4Dx®:
In-vitro diagnostic for the detection of Dirofilaria immitis antigen antibody to Anaplasma phagocytophilum antibody to Borrelia burgdorferi and antibody to Ehrlichia canis in canine serum plasma or whole blood. Precautions and Warnings:
- All wastes should be properly decontaminated prior to disposal.
- Do not mix components from kits with different lot numbers.
- Do not use a SNAP device that has been activated prior to the addition of sample.
Storage:
- Store at 2° - 7°C (35° - °F) until expiration date.
- SNAP devices and reagents can be stored at room temperature (15° - 25°C/59° - 77°F) for 90 days or until the expiration date whichever occurs first.
- After SNAP devices and reagents are removed from 2° - °C (35° - 45°F) for more than 24 hours the expiration date is 90 days or the printed expiration date whichever occurs first.
- If the 90-day expiration date occurs prior to the printed expiration date record the new date in the space provided on the kit.
Kit Components:
Each kit contains one 8-mL bottle of anti-HW/AP/LY/EC:HRPO conjugate and one reagent rack; as well as 5 15 or 30: transfer pipettes sample tubes and SNAP devices. Each SNAP device contains 0.4 mL of wash solution and 0.6 mL of substrate solution. Test Procedure:
| 1. If stored in a refrigerator allow all components to equilibrate at room temperature (15° - 25°C/59° - 77°F) for 30 minutes. Do not heat. | | 2. Using the pipette provided dispense 3 drops of sample into a new sample tube. |  | | 3. Holding the bottle vertical add 4 drops of conjugate to the sample tube. |  | | 4. Cap the sample tube and mix it thoroughly by inverting it 3-5 times. |  | 5. Place the device on a horizontal surface. Add the entire contents of the sampletube to the sample well being careful not to splash the contents outside of the sample well.
The sample will flow across the result window reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well. |  | | 6. When color FIRST appears in the activation circle push the activator firmly until it is flush with the device body. |  |  | | NOTE: Some samples may not flow to the activation circle within 60 seconds and therefore the circle may not turn color. In this case press the activator after the sample has flowed across the result window. | | 7. Read test result at eight minutes. |
Interpreting the Test Results:
Positive Results
Any color development in the sample spots indicates the presence of heartworm antigen
A. phagocytophilum antibody
B. burgdorferi antibody or
E. canis antibody in the sample. |  | | NOTE: Initial research data suggests the SNAP 4Dx Anaplasma phagocytophilum spot could cross react with Anaplasma platys. In studies involving dogs infected with a laboratory strain of A. platys the SNAP 4Dx was reactive with serum from 10 out of 10 infected animals. | Negative Results
Only positive control spot develops color. |  | Invalid Results
- Background-If the sample is allowed to flow past the activation circle background color may result.
Some background color is normal. However if colored background obscures test result repeat the test. - No Color Development-If positive control does not develop color repeat the test.
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